Determination of Morphine and Codeine in Serum after Poppy Seed Consumption Using Gas Chromatography – Mass Spectrometry
نویسنده
چکیده
An analytical method is presented here for the assessment of morphine and codeine concentrations in human serum by employing mass spectrometry in the selective ion monitoring mode following its separation with gas chromatography. Deuterated (marked with heavy water) analogies of the analytes were used as surrogate internal standards. Samples were cleaned up using solid phase extraction. The target compounds were analyzed as pentafluoropropionic esters. The monitored ions were m/z=414, 577 and 361 [morphine], m/z=417, 580 and360 [D3-morphine], m/z=282, 445 [codeine], m/z=274, 448, [D3-codeine]. The recovery of the analytes from solid phase extraction was 70 to 80% (morphine) and 75 to 85% (codeine) at a concentration of 10 ng/mL. The limit of quantitation (confidence limit) was 0.87 ng/mL for morphine and 0.9 ng/mL for codeine. Calibration was accomplished employing blank human serum spiked with the analytes at various concentrations.
منابع مشابه
Concentrationsof Morphineand Codeine in Serum and Unne after Ingestionof Poppy Seeds
We measured morphine and codeine in commercially available poppy seeds and in serum and urine samples from healthy adults who had ingested these poppy seeds. Four brands of black poppy seeds, examined by gas chromatography-mass spectroscopy (GC-MS) with deuterated internal standards, contained from 17 to 294 of morphine and 3 to 14 g of codeine per gram of seeds. Morphine was detected by GC-MS ...
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